Massive parallel analysis of DNA-Hoechst 33258 binding specificity with a generic oligodeoxyribonucleotide microchip
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چکیده
منابع مشابه
Massive parallel analysis of DNA-Hoechst 33258 binding specificity with a generic oligodeoxyribonucleotide microchip.
A generic oligodeoxyribonucleotide microchip was used to determine the sequence specificity of Hoechst 33258 binding to double-stranded DNA. The generic microchip contained 4096 oxctadeoxynucleo-tides in which all possible 4(6)= 4096 hexadeoxy-nucleotide sequences are flanked on both the 3'- and 5'-ends with equimolar mixtures of four bases. The microchip was manufactured by chemical immobiliza...
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Spectrophotometric study of Hoechst 33258 binding to DNA has been investigated. It was shown that this ligand forms at least two types of complexes at 0.002 M and 0.02M ionic strengths of solution. One of them is strong, the other is weak. It was revealed that at 0.02M ionic strength of solution, the binding constant value by strong mode is one order higher than at 0.002M. It was obtained that ...
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In this work the data of methylene blue (MB) and Hoechst 33258 interaction with DNA are presented. It has been shown that at 0.002 M ionic strength of solution MB is bound to double-stranded DNA by three modes: intercalation, semi-intercalation and electrostatic. It was revealed that at higher ionic strengths of solution complete intercalation of MB becomes thermodynamically unacceptable. It wa...
متن کاملMassive parallel analysis of the binding specificity of histone-like protein HU to single- and double-stranded DNA with generic oligodeoxyribonucleotide microchips.
A generic hexadeoxyribonucleotide microchip has been applied to test the DNA-binding properties of HU histone-like bacterial protein, which is known to have a low sequence specificity. All 4096 hexamers flanked within 8mers by degenerate bases at both the 3'- and 5'-ends were immobilized within the 100 x 100 x 20 mm polyacrylamide gel pads of the microchip. Single-stranded immobilized oligonucl...
متن کاملQuantitation of DNA using Hoechst 33258
An essential element of cellular and molecular biology is the ability to quantitate DNA in large numbers of samples at a sensitivity that enables determination of small amounts of sample. Here we describe a fluorescent method to quantitate DNA with Hoechst 33258 dye and the BioTek FL600 fluorescence microplate reader. This assay provides linear results over three orders of magnitude and can rou...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1999
ISSN: 1362-4962
DOI: 10.1093/nar/27.20.4100